Bacteria drops to 23%, 2.2% and 0% (2-4-6 minutes treatment with O3 Nanobubble)
Study suggests further investigation of O2 Nanobubble and short-term O3 Nanobubble on live shrimps
This study investigated the ability of nanobubbles to reduce Vibrio parahaemolyticus (AHPND strain) and to improve water quality. Two laboratory experiments were conducted over a one-week period, that is (a) assessing the effects of air and oxygen nanobubbles for 60 minutes per day and (b) comparing effects of ozone nanobubble treatments for 2, 4 and 6 minutes per day. Experiments were done in triplicate 100 L tanks with 15‰ saline water, inoculated with an initial bacterial concentration of 106 CFU/ml. At the end of experiment 1, the bacterial concentration of the air and oxygen nanobubble groups was counted for 69% and 46% of the control group respectively. At the end of experiment 2, the bacterial concentration of the 2-, 4- and 6-minute ozone nanobubble groups were counted for 23%, 2.2% and 0% of the control group respectively. Oxygen and ozone nanobubbles significantly increased oxygen reduction potential and oxygen values. Results indicate that under effective dosages nanobubbles can be used in the production farms to control V.parahaemolyticus and increase oxygen levels.
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The study evaluated the ability of NB gases to reduce the level of V.parahaemolyticus(AHPND strain) in an experimental setting. With an initial bacterial concentration of 106 CFU/ml, after one-week period, bacteria counts in the air and oxygen NB treatments were 69% and 46% of that in the control group. Bacteria counts in the 2, 4 and 6 minutes of ozone NBs treatments experiment were 23%, 2.2% and 0% of that in the control group. The use of NB treatments demonstrated the reduction in bacterial growth, especially ozone NB treatments. In addition, oxygen and ozone NBs significantly increased dissolved oxygen levels to more than 20 mg/L, which is beneficial for aquatic animals if this technology is used in aquaculture. From the present results, we propose to further investigate the safety of using oxygen NB and short exposure times with ozone NBs on live shrimp to determine whether it can be used to control disease outbreaks. We also caution that water quality parameters such as temperature and pH should be closely monitored when NBs are applied in systems with relatively small volumes.
