Nanobubbles (<200 nm in diameter) have several unique properties such as
long lifetime in liquid owing to its negatively charged surface, and its high
gas solubility into the liquid owing to its high internal pressure. They are used
in variety of fields including diagnostic aids and drug delivery, while there are
no reports assessing their effects on the growth of lives. Nanobubbles of air or
oxygen gas were generated using a nanobubble aerator (BUVITAS; Ligaric Company
Limited, Osaka, Japan). Brassica campestris were cultured hydroponically for
4 weeks within air-nanobubble water or within normal water. Sweetfish (for 3
weeks) and rainbow trout (for 6 weeks) were kept either within air-nanobubble
water or within normal water. Finally, 5 week-old male DBA1/J mice were bred
with normal free-chaw and free-drinking either of oxygen-nanobubble water or
of normal water for 12 weeks. Oxygen-nanobubble significantly increased the
dissolved oxygen concentration of water as well as concentration/size of nanobubbles
which were relatively stable for 70 days. Air-nanobubble water significantly promoted
the height (19.1 vs. 16.7 cm; P<0.05), length of leaves (24.4 vs. 22.4 cm; P<0.01), and
aerial fresh weight (27.3 vs. 20.3 g; P<0.01) of Brassica campestris compared to normal water.
Total weight of sweetfish increased from 3.0 to 6.4 kg in normal water, whereas it
increased from 3.0 to 10.2 kg in air-nanobubble water. In addition, total weight of
rainbow trout increased from 50.0 to 129.5 kg in normal water, whereas it increased
from 50.0 to 148.0 kg in air-nanobubble water. Free oral intake of oxygen-nanobubble
water significantly promoted the weight (23.5 vs. 21.8 g; P<0.01) and the length
(17.0 vs. 16.1 cm; P<0.001) of mice compared to that of normal water. We have
demonstrated for the first time that oxygen and air-nanobubble water may be potentially
effective tools for the growth of lives
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